Journal of Radio Electronics. eISSN 1684-1719. 2025. ¹6

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DOI: https://doi.org/10.30898/1684-1719.2025.6.13

 

 

 

STUDY OF PHOTOCYTOTOXICITY
AND DARK CYTOTOXICITY OF THE Yb COMPLEX
OF 2,4-DI(α-METHOXYETHYL)DEUTEROPORPHYRIN IX
for biomedical photonics purposes

 

I.P. Shilov1, Yu.V. Alekseev2, A.V. Ivanov2,4, V.D. Rumyantseva1,3

 

1IRE Kotelnikov RAS, Fryazino branch,
141190, Russia, Fryazino, Vvedenskogo Sq. 1

2«Skobelkin Scientific and Practical Center of Laser Medicine» FMBA of Russia,
121165, Russia, Moscow, Studencheskaya st., 40

3MIREA – Russian Technological University,
119454, Russia, Moscow, Vernadskogo Ave., 78

4«N.N. Blokhin National Medical Research Center of Oncology» of the Russian Ministry of Health,
115522, Russia, Moscow, Kashirskoe highway, 23

 

The paper was received March 18, 2025.

 

Abstract. The photocytotoxicity and dark toxicity of the Yb complex of 2,4-di(α-methoxyethyl)deuteroporphyrin IX (Yb-DMDP) were studied to determine the prospects for its further application in biomedical photonics. The parameters of Yb-DMDP intrinsic cytotoxicity (dark) and photocytotoxicity were determined using the MTT assay. For dark cytotoxicity, the 50% survival rate of cancer cells (IC50) was 10−5.15 mol/L for Skov-3 cells, and for phototoxicity, the IC50 was 10−5.53 mol/L. Thus, it has been shown that the Yb-DMDP compound has moderate intrinsic (dark) toxicity and insignificant photocytotoxicity compared to the drug «Photoditazine», which is widely used in clinical practice. Low photocytotoxicity will allow the Yb-DMDP effective use in the processes of IR-luminescent diagnostics of tumors, and moderate dark cytotoxicity, apparently, can make it possible to use it in antimicrobial therapy without photoexcitation.

Key words: photocytotoxicity, dark cytotoxicity, ytterbium porphyrin complex, MTT assay, cell irradiation stand, photosensitizers, photodynamic effect.

Financing: The work was carried out according to the State Assignment of the V.A. Kotelnikov IRE RAS (¹ FFWZ-2025-0013) and with the financial support of the Ministry of Science and Higher Education of the Russian Federation (project No. FSFZ-2023-0004).

Corresponding author: Shilov Igor Petrovitch, laserlab@ms.ire.rssi.ru

References

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For citation:

Shilov I.P., Alekseev Yu.V., Ivanov A.V., Rumyantseva V.D. Study of photocytotoxicity and dark cytotoxicity of the Yb complex of 2,4-di(α-methoxyethyl)deuteroporphyrin IX for biomedical photonics purposes. // Journal of Radio Electronics. – 2025. – ¹. 6. https://doi.org/10.30898/1684-1719.2025.6.13 (In Russian)