Abstract.
For the first time, new nanocomposite liposomes and
vesicles with a modified structure containing functional inorganic electrically
conductive nanoparticles associated with both the inner and the outer surface
of the liposomal membrane were prepared. The effect of ultrashort electric
field pulses with a duration of less than 10 ns and an intensity in a
dielectric aqueous medium of the order of 10 kV/cm on the aqueous suspension of
such nanocomposite liposomes containing an encapsulated model low molecular
weight compound (NaCl) has been studied. It was found that as a result of
exposure to such impulses, decapsulation and destruction of nanocomposite
liposomes present in the suspension occurs, accompanied by a corresponding
increase in the conductivity of the suspension. It is shown that the
sensitivity of nanocomposite liposomal capsules to external electrical effects
is due to the inclusion of electrically conductive nanoparticles in their
structure. It was found that the effect of decapsulation of liposomal capsules
is significantly higher in the case of the impact of electric field pulses on
liposomal capsules with bound magnetite nanoparticles compared with the case of
a similar effect on the same capsules that do not contain conducting
nanoparticles. This fact determines the selectivity of the effect of electric
pulses on nanocomposite membrane vesicles containing electrically conductive
nanoparticles. A theoretical analysis of non-thermal interaction of
nanostructured liposomal capsules containing conducting nanoparticles on the
outer and inner surfaces of the membrane with ultrashort electrical pulses was
carried out. A theoretical model of non-thermal interaction of nanostructured
liposomal capsules with ultrashort electrical pulses is constructed. In the
model under consideration, electrically conductive nanoparticles are associated
with the outer and inner surfaces of membranes of nanocomposite liposomal
capsules. Within the framework of the constructed model, the mechanisms of
destruction of the liposomal capsule membrane are described, due to the
interaction of conducting spherical nanoparticles located on opposite surfaces
of the liposomal membrane resulting from the ultrashort electrical effect on
aqueous suspensions of nanostructured liposomal capsules.
Keywords:
capsules, liposomes,
structure, nanoparticles, polyelectrolytes, ultrashort pulse of electric field.
References
1. Donath E., Sukhorukov G.B., Caruso F., Devis S.A., Möhwald H.
Novel Hollow Polymer Shells by Colloid-Templated Assembly of Polyelectrolytes. Angew
Chem. Int. Ed. Engl., 1998, Vol. 37, p. 2202.
2. Sukhorukov G.B., Donath E., Davis S.A., Lichtenfeld A.,
Caruso F., Popov V.I., Möhwald H. Stepwise polyelectrolyte assembly on particle
surfaces: A Novel Approach to Colloid Design. Polym. Adv. Technol.,
1998, Vol. 9, p. 759.
3. Sukhorukov G.B., Antipov A., Voigt A., Donath E., Möhwald H.
pH-Controlled Macromolecule Encapsulation in and Release from Polyelectrolyte
Multilayer Nanocapsules. Macromol. Rapid Commun, 2001, Vol. 22, pp.
44-46.
4. Gennady B. Khomutov, Vitaly P. Kim, Kirill V.
Potapenkov, Alexander A. Parshintsev, Eugene S. Soldatov, Nazym N. Usmanov,
Alexander M. Saletsky, Andrey V. Sybachin, Alexander A. Yaroslavov, Vasiliy A.
Migulin, Igor V. Taranov, Vladimir A. Cherepenin, Yury V. Gulyaev, Langmuir
monolayers and Langmuir-Blodgett films of pH-sensitive lipid. Colloids and
Surfaces A: Physicochemical and Engineering Aspects, 2017, Vol. 532,
pp. 150-154.
5. Radt B., Smith T.A., Caruso F. Optically Addressable
Nanostructured Capsules. Adv. Mater, 2004, vol. 16, pp. 2184-2189.
6. Lu Z., Prouty M.D., Guo Z., Golub V.O., Kumar C.S.S.R., Lvov
Y.M. Magnetic switch of permeability for polyelectrolyte microcapsules embedded
with Co, Au nanoparticles. Langmuir, 2005, Vol. 21, pp. 2042-2050.
7. D. A. Gorin, D. G. Shchukin, A. I. Mikhailov, K.
Köhler, S. A. Sergeev, S. A. Portnov, I. V. Taranov, V. V. Kislov, G. B.
Sukhorukov. Effect of Microwave Radiation on Polymer Microcapsules Containing
Inorganic Nanoparticles. Technical Physics Letters, 2006, Vol. 32, No.
1, pp. 70–72.
8. Gorin D.A., Shchukin D.G., Koksharov Yu.A.,
Portnov S.A., Köhler K., Taranov I.V., Kislov V.V., Khomutov G.B., Möhwald H.,
Sukhorukov G.B. Effect of microwave irradiation on composite iron oxide
nanoparticle/polymer microcapsules. Progress in Biomedical Optics and
Imaging. 2007. Vol. 6536, p. 653604.
9. Gulyaev Y.V., Cherepenin V.A., Vdovin V.A.,
Taranov I.V., Sukhorukov G.B., Gorin D.A., Khomutov G.B. Decapsulation of
polyelectrolyte nanocomposite microcapsules by pulsed microwave effect. Journal
of Communications Technology and Electronics, 2015. Vol. 60, No. 11, pp.
1286-1290.
10. Schwendener R.A. Bio-Applications of Nanoparticles.
Edited by Chan W.C.W. Ser. Advances in Experimental Medicine and Biology,
2007, Vol. 620, p. 117.
11. Amstad E., Kohlbrecher J., Muller E., Schweizer T.,
Textor M., Reimhult E. Triggered Release from Liposomes through Magnetic
Actuation of Iron Oxide Nanoparticle Containing Membranes. Nano Letters,
2011, Vol. 11, pp. 1664- 1670.
12. Yu. V. Gulyaev, V. A. Cherepenin, I. V. Taranov, V.
A. Vdovin, A. A. Yaroslavov, V. P. Kim, G. B. Khomutov. Remote decapsulation of
nanocomposite liposomal capsules containing gold nanorods by ultrashort
electric pulses. Journal of Communications Technology and Electronics, 2016,
Vol. 61, No. , pp. 56-60.
13. Gulyaev Y.V., Cherepenin V.A., Vdovin V.A., Taranov
I.V., Khomutov G.B., Yaroslavov A.A., Kim V.P. Pulsed electric field-induced
remote decapsulation of nanocomposite liposomes with implanted conducting
nanoparticles. Journal of Communications Technology and Electronics. 2015.
Ò. 60. ¹
10. Ñ. 1097-1108.
14. G B. Khomutov, V P. Kim, Y A., Koksharov, K V.
Potapenkov, A A. Parshintsev, E S. Soldatov, N N. Usmanov, A M., S, A V.
Sybachin, A A. Yaroslavov, I V. Taranov, V A. Cherepenin, Y V. Gulyaev.
Nanocomposite biomimetic vesicles based on interfacial complexes of
polyelectrolytes and colloid magnetic nanoparticles. Colloids and Surfaces
A: Physicochemical and Engineering Aspects, 2017, Vol. 532, 5 November, pp.
26-35.
15. Gubin S.P., Gulyaev Yu.V., Khomutov G.B., Kislov V,
V., Kolesov V, V., Soldatov, E.S., Sulaimankulov, K.S., Trifonov, A.S. Molecular clusters as building blocks for nanoelectronics:
The first demonstration of a cluster single-electron tunnelling transistor at
room temperature. Nanotechnology. 2002, Vol. 13, No. 2, pp.185.
16. Kislov V.V., Kolesov V.V., Taranov I.V. Electron
transport through a molecular nanocluster. Journal of Communications
Technology and Electronics, 2002, Vol. 47, No. 11, pp. 1266-1270.
17. Yu.V. Gulyaev, V.Kislov, V.V.Kolesov, I.V.Taranov,
S.P.Gubin, G.B.Khomutov, E.S.Soldatov, I.A.Maximov, L.Samuelson. Electronics
of Molecular Nanoclusters. International Journal of Nanoscience, 2004, Vol.
3, No. 1-2 pp. 137-147.
18. Kislov V., Medvedev B., Gulyaev Yu., I. Taranov and V.
Kashin. Organized superstructures at nanoscale and new functional
nanomaterials.
International Journal of Nanoscience. 2007, Vol. 6, No.
5,
pp. 373.
19. Koning G.A., Eggermont A.M.M., Lindner L.H., ten
Hagen T.L.M. Pharmaceutical Research, 2010, Vol. 27, No. 8, pp. 1750.
20. Artemyev M., Kisiel D., Abmiotko S. M. N. Antipina, G. B. Khomutov,
V.V. Kislov, A.A. Rakhnyanskaya. Self-Organized,
Highly Luminescent CdSe Nanorod-DNA Complexes. Journal American Chemical Society, 2004, Vol. 126, No. 34,
pp. 10594.
21. Massart R. Preparation of aqueous magnetic liquids in alkaline and
acidic media. IEEE Transactions on Magnetics, 1981, Vol. 17, pp. 1247−1248.
22. L. D. Landau, E. M. Livshits, Electrodynamics of
Continuous Media. Pergamon, Oxford, 1984.
23. V. P. Kim, A. V. Ermakov, E. G. Glukhovskoy, A. A.
Rakhnyanskaya, Yu. V. Gulyaev, V. A. Cherepenin, I. V. Taranov, P. A.
Kormakova, K. V. Potapenkov, N. N. Usmanov, A. M. Saletsky, Yu. A. Koksharov, G.
B. Khomutov. Planar Nanosystems on the Basis of Complexes Formed by
Amphiphilic Polyamine, Magnetite Nanoparticles, and DNA Molecules Nanotechnologies
in Russia, 2014, Vol. 9, No. 5–6, pp. 280–287.