HEMOCYTOMETER-BASED
SIMPLE MORPHOMETRIC LAB-ON-A-CHIP WITH RF - IDENTIFICATION & TRANSLATION OF CELL CULTURE
HYSTOCHEMICAL MONITORING DATA
A.
V. Notchenko 1,2, J.
V. Gradov 1,3
1
Neuroscience Research Center RAMS; Brain Research Department;
Brain Neural Structure Laboratory
2
Bauman Moscow State Technical University
3
Institute of Chemical Physics RAS
Received
January 20, 2012
Abstract. An accessible design of
autonomous labs on the chip which do not require a special reader is developed.
The proposed device uses hemocytometric counting chambers for determination of
concentration of cells, isolated by a special device for cell sedimentation. A
system of automated RF-identification of chambers in the long-term storage is
proposed which provides new morphometric data at various stages of cultivation
or biomonitoring. A new diffraction method of calculation and fingerprinting of
cellular structures in varying environmental conditions is described. Experimental
data on the pilot testing of Fuchs-Rosenthal, Buerker, Neubauer, Makler and
Thoma chambers for the described technique is given. The applicability of these
devices for coherent-densitometric indication either of concentration dynamics
in cell culture or suspension or morphogenetic processes in them for laminar
conditions is shown. A self-learning program for cellular pattern recognition
and relational cytophotometry based on lab on the chip with an
object-micrometric calibration relative to the counting net is demonstrated. A
hybrid in vitro / in silico method of morphogenesis monitoring in cell cultures
is introduced.
Keywords: lab-on-a-chip,
telemedicine, CCD, CMOS, RFID, mathematical morphology, spectrozonal mapping, machine
learning, machine vision, pattern recognition, automatic classification, hystochemistry.