"JOURNAL OF RADIO ELECTRONICS" (Zhurnal Radioelektroniki)  ISSN 1684-1719, N 12, 2016

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RESEARCH OF NEW FLUOROCARBON EMULSION BY 19F MRI AND 19F NMR SPECTROSCOPY IN COMPARISON WITH THE DRUG «PERFTORANUM®» AT STRONG MAGNETIC FIELD 7 T

 

M. V. Gulyaev 1, A. V. Kuznetsova 1, L. L. Gervits 2, Yu. A. Pirogov 1

1 Lomonosov Moscow State University

2 Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences

 

The paper is received on December 1, 2016

 

Abstract. The first fluorocarbon emulsion synthesized from perfluorocarbons (PFCs) and used in clinical medicine in Russia was the drug Perftoranum® - blood substitute with an effective gas transportation function. Despite of the fact that its composition contains a large number of fluorine-19 nuclei, the contribution to the resultant 19F NMR signal in a strong magnetic field is given by only small part of the summery amount of fluorine nuclei – because chemical shifts are increased in a high magnetic field, it is necessary to use the selective excitation method. In addition, most of these nuclei are magnetically nonequivalent, so it is also problematically to obtain 19F MR images – chemical shift artifacts may appear. As a result, it requires quite a lot of time – at least 20 minutes for obtaining acceptable quality 19F MR images of the drug Perftoranum®. The presence of magnetically equivalent nuclei of fluorine-19 in a substance greatly simplifies obtaining its 19F MR images. In this case, on the 19F spectrum NMR will be observed a single intense peak that creates favorable conditions for an MRI scanning – the signal/noise ratio that allows obtaining 19F MR images with higher contrast in less time will be significantly increased. The presence of 9 and 12 magnetically equivalent fluorine-19 nuclei at the mixture of two oligomeric monobromide perfluorinated ethers forms such singlet spectral peak. With help of 19F NMR and 19F MRI experiments in vivo, this paper studies a  new fluorocarbon emulsion that has been synthesized on the base of mixing mentioned substance and perfluorodecalin. The presence of a single intense singlet on its 19F NMR spectrum allows obtaining 19F MR images of the quality comparable with the drug Perftoranum® one but in 2.5 times faster than for the Perftoranum® with the same scanning parameters. By NMR spectroscopy in vivo with laboratory animals (rats Wistar), studies on determining dynamics of the PFCs removal from animal organism in the case of the new emulsion were carried out. It was shown that the half-life time of perfluorodecalin removal is approximately 7 days, while during the significantly longer observation time (14 days), the amount of fluorine-19 nuclei in the mixture of two oligomeric monobromide perfluorinated ethers virtually unchanged.

Keywords: 19F NMR spectroscopy, 19F MRI, perfluorocarbon emulsion, Perftoranum®.

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