MAGNETIC RESONANCE THERMOMETRY OF RATS BRAIN BY LOCAL NMR SPECTROSCOPY METHOD

 

M. V. Gulyaev ¹, S. K. Khanov ¹, D. D. Namestnikova ², L. V. Gubskiy ², Jaw Fushan ³, Yu. A. Pirogov ¹, V. Ya. Panchenko ¹

¹ M. V. Lomonosov Moscow state university

² N. I. Pirogov Russian national research medical university

³ National university of Taiwan

The paper is received on October 16, 2013

Abstract. It is developed a method measuring temperature of internal tissues in the area of artificially created cerebral ischemia for rats by NMR-spectroscopy. NAA (N-acetylaspartate), Cho (choline), Cr (creatine) metabolites were selected as reference values ​​for these measurements. In vivo experiments on laboratory animals with local ¹H NMR spectroscopy showed that based on the measurement of the chemical shift of the spectral lines by using the amplitude-frequency NMR spectrum one can define a metabolic portrait and the temperature of interesting tissue. So the proton NMR spectroscopy in combination with MRI localization of measured voxel (local NMR spectroscopy) can serve as an effective way of non-invasive in vivo measuring the brain tissues temperature and determination of ischemia extent.

Key words: magnetic resonance thermometry, local NMR spectroscopy, cerebral ischemia, metabolites, magnetic resonance imaging.