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Journal of Radio Electronics Online scientific journal · eISSN 1684-1719
2023, № 12 · BIOMEDICAL RADIO ELECTRONICS

Magnetite nanoparticles increase the conductivity of azolectin bilayer in an inhomogeneous magnetic field

A.A. Anosov1, E.D. Borisova1, I.V. Taranov1, I.V. Grigoryan1, V.A. Cherepenin1, G.B. Khomutov1

1 Kotelnikov IRE RAS, Moscow, Russia
Full text DOI: 10.30898/1684-1719.2023.12.5 UDC 577.352.26 EDN NZGPGY

Abstract

In the magnetofection method, magnetic fields and magnetic nanoparticles are used to increase the efficiency of gene delivery into cells. Magnefection enhances the introduction into cells of gene vectors with which magnetic nanoparticles are associated, due to the action of a magnetic field that holds the nanoparticles in the area of their application. It is believed that the magnetic field itself does not change the mechanism of absorption (endocytosis) of nanoparticles. Both the beneficial effect of magnetofection - delivery of the vector into the cell, and its side effect - cytotoxicity are associated with the interaction of particles with cell membranes and, in particular, with lipid bilayers. In our work, we investigated the effect of an applied stationary inhomogeneous magnetic field and spherical superparamagnetic magnetite nanoparticles with a diameter of about 4 nm on the conductivity of azolectin bilayer lipid membranes. The membranes were formed in a stationary magnetic field with a magnetic induction of up to 26 mT. The magnetic field had no effect on the conductivity of the membrane. After monitoring the membrane conductivity, magnetic nanoparticles were added to the solution surrounding the membrane. The addition was carried out on one side of the membrane so that the magnetic field attracted nanoparticles to the membrane surface. After adding nanoparticles in a magnetic field, the conductivity of the membranes increased by one to two orders of magnitude. This effect was observed for all membranes. A smooth increase in conductivity was accompanied in a number of cases (for 25% of the membranes) by the appearance of current jumps, which can be associated with the formation of through conducting pores with a radius of about 0.5 nm. The conductivity increased with increasing magnetic field gradient.

  • Magnetofection
  • magnetite nanoparticles
  • stationary magnetic field
  • Membrane conductivity
Аннотация (на русском)

Наночастицы магнетита увеличивают проводимость азолектинового бислоя в неоднородном магнитном поле

В методе магнитофекции магнитные поля и магнитные наночастицы используются для повышения эффективности доставки генов в клетки. Магнитофекция усиливает внедрение в клетки генных векторов, с которыми связаны магнитные наночастицы, за счет действия магнитного поля, которое удерживает наночастицы в области их применения. При этом считается, что само магнитное поле не меняет механизм поглощения (эндоцитоза) наночастиц. Как полезный эффект магнитофекции - доставка вектора в клетку, так и ее побочный эффект - цитотоксичность связаны с взаимодействием частиц с клеточными мембранами и, в частности, с липидными бислоями. В нашей работе исследовано влияние приложенного стационарного неоднородного магнитного поля и сферических суперпарамагнитных наночастиц магнетита диаметром около 4 нм на проводимость азолектиновых бислойных липидных мембран. Мембраны формировали в стационарном магнитном поле с магнитной индукцией до 26 мТл. Магнитное поле никак не влияло на проводимость мембраны. После контроля мембранной проводимости в окружающий мембрану раствор добавляли магнитные наночастицы. Добавка проводилась с одной стороны мембраны таким образом, чтобы магнитное поле притягивало наночастицы к поверхности мембраны. После добавления наночастиц в магнитном поле проводимость мембран увеличивалась на один-два порядка. Этот эффект проявлялся для всех мембран. Плавное увеличение проводимости сопровождалось в ряде случаев (для 25 % мембран) появлением скачков тока, что можно связать с образованием сквозных проводящих пор радиусом около 0.5 нм. Проводимость увеличивалась с увеличением градиента магнитного поля.

For citation

Anosov A.A., Borisova E.D., Taranov I.V., Grigoryan I.V., Cherepenin V.A., Khomutov G.B. Magnetite nanoparticles increase the conductivity of azolectin bilayer in an inhomogeneous magnetic field // Journal of Radio Electronics. – 2023. – No. 12. https://doi.org/10.30898/1684-1719.2023.12.5

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